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Home >> Antibodies >> Recombinant MSLN Antibody / Mesothelin

Recombinant MSLN Antibody / Mesothelin [clone rMSLN/8764] (V4429)

  Catalog No Formulation Size Price (USD)  
Image V4429-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4429-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4429SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human ovarian cancer tissue with recombinant MSLN antibody (clone rMSLN/8764). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant MSLN antibody (clone rMSLN/8764) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rMSLN/8764
Purity Protein A/G affinity
UniProt Q13421
Localization Cell Surface, Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant MSLN antibody is available for research use only.
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Description

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Application Notes

Optimal dilution of the recombinant MSLN antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 273-407) from the human protein was used as the immunogen for the recombinant MSLN antibody.

Storage

Aliquot the recombinant MSLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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